YAP1 Antibody - CD BioSciences

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YAP1 Antibody

YAP1 Antibody

SPA-11888

Size Price
25 µg Online Inquiry
100 µg Online Inquiry
More Options Online Inquiry
Target Information
Target Name YAP
Gene Abbr. YAP1
Gene ID 10413
Full Name Yes1 associated transcriptional regulator
Alias COB1, YAP, YAP2, YAP65, YKI
Introduction YAP (Yes-associated protein, YAP65) was first identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl. In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains. While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors. In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size. Phosphorylation at multiple sites (e.g., Ser109, Ser127) by LATS kinases promotes YAP translocation from the nucleus to the cytoplasm, where it is sequestered through association with 14-3-3 proteins. These LATS-driven phosphorylation events serve to prime YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron, triggering proteosomal degradation of YAP.TAZ is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 proteins. TAZ shares homology with the WW domain of Yes-associated protein (YAP). TAZ is proposed to modulate the switch between proliferation and differentiation of mesenchymal stem cells (MSC) via interaction with transcription factors Runx2 and PPARγ. This process is critical to normal tissue development and the prevention of tumor formation. Due to its role in determination of MSC fate, TAZ may have clinical relevance to several human diseases caused by an imbalance of MSC differentiation. TAZ is negatively regulated via phosphorylation by LATS1/2, core kinases in the Hippo signaling pathway that controls stem cell development, tissue growth and tumor development.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. 867711
Isotype IgG1
Immunogen E. coli-derived recombinant human YAP1. Ala347-Leu504. P46937.
Usage
Application WB, IHC
Dilutions Western Blot (2 µg/mL); Immunohistochemistry (8-25 µg/mL)
MW(KDa) 65-78
Reactivity Human, Mouse, Rat
Validation Knockout Validated.
Specificity Detects human YAP1 in ELISA. Detects human, mouse and rat YAP1 in Western Blots.
Storage & Handling
Storage Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative No Preservative
Storage Temp. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Handling Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.