Acetyltransferases & Deacetylases - CD BioSciences

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Acetyltransferases & Deacetylases

Acetyltransferases & Deacetylases

Acetylation is one of the major protein modifications in cells, where the acetyl group from acetyl coenzyme A (acetyl-CoA) is transferred to a polypeptide chain. Protein acetylation can occur at N-terminal as a co-translational covalent modification by N-terminal acetyltransferases (NATs), or on lysine as a reversible post-translational modification (PTM). Like phosphorylation, acetylation also affects protein function by altering the properties of proteins such as hydrophobicity, solubility, protein conformation and affinity with other macromolecules.

Thousands of acetylation have been identified in human proteome, among which chromatin proteins and metabolic enzymes are highly represented such as histones, P53, and STAT3. Consequently, dysregulation of acetylation can lead to metabolic disorders, cancers, neurodegeneration, and cardiovascular diseases.

CD BioSciences offers a complete portfolio of solutions to study protein acetylation including acetylation profiling, acetyltransferase/deacetylase identification, substrate identification, and inhibitor/activator screening.

acetyltransferases and deacetylases

Acetyltransferase and Acetylation

Examples: Histone acetyltransferases (HATs, e.g., p300/CBP), N-terminal acetyltransferases (NATs)

Acetyltransferases are the enzymes carrying out acetylation where an acetyl group from acetyl-CoA is transferred to a protein. In epigenetics, histone acetylation/deacetylation is linked to transcriptional regulation of gene expression. Malfunction of histone acetylation/deacetylation machinery is linked to diseases such as cancers, inflammatory diseases (leukemia) to neurological disorders (fragile X syndrome). There are other proteins with important roles in the signaling pathway, whose activity is also regulated by acetylation, such as P53, Tubulins and STAT3. And other relevant diseases include cardiovascular disease and metabolic disorders.

Deacetylase and Deacetylation

Examples: Histone deacetylases (HDACs), Sirtuins (SIRT1,2,3,5).

Deacetylases are enzymes carrying out deacetylation where an acetyl group is removed from lysine amino acids in proteins. HDACs are the histone deacetylases which regulate gene expressions by modifying how tight DNA is wrapped. HADCs can also deacetylate non-histone proteins such as transcription factors (e.g., NF-κB) and co-regulators. The tumor suppressor PTEN can be deacetylated by SIRT1 and HADC1, thus gaining activities. With a crucial role in various diseases, HADC inhibitors/ histone deacetylase inhibitors (HDIs) are potential treatments for cancers, interstitial fibrosis, autoimmune and inflammatory diseases, neurodegenerative diseases and metabolic disorders.

Solutions for Acetyltransferases & Deacetylases

CD BioSciences is dedicated to providing personalized solutions for you to study your acetyltransferases/deacetylases and related signaling pathways.

acetyltransferases and deacetylases

Acetylation Profiling

  • Profiling global protein acetylation in a system (cells, tissues, etc).
  • Detecting acetylation of proteins in specific signaling pathways.
  • Determining the ratio of acetylated protein vs. unacetylated protein.
acetyltransferases and deacetylases

Histone Acetylation Profiling

  • Determining the loci (DNA motif) in the genome with histone acetylation by genome-wide histone acetylation profiling.
  • Comparing gene expressions with/without acetylation on certain histone sites (e.g. histone H4 (K5, K8, K12 and K16), histone H3 (K9, K14, K18, K23 and K27)).
acetyltransferases and deacetylases

Substrate Identification

  • Identifying substrates/histones for acetyltransferases or deacetylases.
  • Determining the acetylation sites on the substrates/histones.
acetyltransferases and deacetylases

Acetyltransferases/Deacetylases Identification

  • Identifying acetyltransferases/deacetylases modifying proteins of interest.
  • Determining the catalytic site on acetyltransferases or deacetylases.
  • Analyzing the structures of acetyltransferases or deacetylases.
acetyltransferases and deacetylases

Inhibitor/Activator Screening

  • Screening for inhibitors or activators for acetyltransferases or deacetylases.
  • Determining the thermodynamic parameters of the interactions.

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For research use only. Not intended for any clinical use.