UBE1a Antibody - CD BioSciences

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UBE1a Antibody

UBE1a Antibody

SPA-11597

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Target Information
Target Name UBE1
Gene Abbr. UBE1
Gene ID 7317
Full Name ubiquitin like modifier activating enzyme 1
Alias A1S9, A1S9T, A1ST, AMCX1, CFAP124
Introduction Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the ubiquitin-activating enzyme (UBE1 or E1). The activated ubiquitin is subsequently transferred to the ubiquitin-carrier protein E2, and then from E2 to ubiquitin ligase E3 for final delivery to the ε-amino group of the target protein lysine residue. Combinatorial interactions of different E2 and E3 proteins result in substrate specificity. UBE1 has two isofoms: UBE1a is a nuclear protein of 117 kDa while UBE1b is a nuclear and cytoplasmic protein of 110 kDa.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Immunogen Synthetic peptide corresponding to the amino terminus of human UBE1.
Source/Purification Antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of human UBE1. Antibodies are purified by protein A and peptide affinity chromatography.
Usage
Application WB, IP, IHC, IF, FC
Dilutions Western Blotting (1:1000); Immunoprecipitation (1:50); Immunofluorescence/Immunocytochemistry (1:50); Flow Cytometry (1:100)
MW(KDa) 117
Reactivity Human, Mouse, Rat
Sensitivity Endogenous
Specificity Detects endogenous levels of total UBE1a protein. This antibody does not cross-react with UBE1b.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.