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TIGAR Antibody
SPA-10772
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | TIGAR |
| Gene Abbr. | TIGAR |
| Gene ID | 57103 |
| Full Name | TP53 induced glycolysis regulatory phosphatase |
| Alias | C12orf5, FR2BP |
| Introduction | The p53 tumor suppressor protein regulates the cellular response to multiple stresses, including DNA damage and oxidative stress. Activation of p53 can lead to cell cycle arrest and DNA repair, or apoptosis. Activated p53 transcription factor regulates the expression of multiple genes that regulate cell metabolism and the cell cycle. One p53-inducible gene is C12orf5, which encodes for a fructose-2,6-bisphosphatase known as TIGAR. TP53-inducible glycolysis and apoptosis regulator (TIGAR) protects cells from oxidative stress as it negatively regulates glycolysis and reduces the production of reactive oxygen species (ROS) in cells. Research studies demonstrate that knockdown of TIGAR expression induces autophagy and apoptosis, and its expression protects cells from ROS-related cell death. Additional studies show that TIGAR promotes cell cycle arrest and supports dephosphorylation of the retinoblastoma (Rb) protein. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D3F4A |
| Immunogen | Synthetic peptide corresponding to residues surrounding Pro171 of human TIGAR protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro171 of human TIGAR protein. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 29 |
| Reactivity | Human |
| Sensitivity | Endogenous |
| Specificity | This antibody recognizes endogenous levels of total TIGAR protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.