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TBC1D1 Antibody
SPA-10639
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | TBC1D1 |
| Gene Abbr. | TBC1D1 |
| Gene ID | 23216 |
| Full Name | TBC1 domain family member 1 |
| Alias | TBC, TBC1 |
| Introduction | TBC1D1 is a paralog of AS160 and both proteins share about 50% identity. TBC1D1 was shown to be a candidate gene for severe obesity. It plays a role in Glut4 translocation through its GAP activity. Studies indicate that TBC1D1 is highly expressed in skeletal muscle. Insulin, AICAR, and contraction directly regulate TBC1D1 phosphorylation in this tissue. Three AMPK phosphorylation sites (Ser231, Ser660, and Ser700) and one Akt phosphorylation site (Thr590) were identified in skeletal muscle. Muscle contraction or AICAR treatment increases phosphorylation on Ser231, Ser660, and Ser700 but not on Thr590; insulin increases phosphorylation on Thr590 only. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic peptide corresponding to the sequence around Gly 689 of human TBC1D1. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence around Gly 689 of human TBC1D1. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 160 |
| Reactivity | Mouse |
| Sensitivity | Endogenous |
| Specificity | This antibody detects endogenous levels of total TBC1D1 protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.