Sumo1 cDNA ORF Clone, Rat, C-Myc tag - CD BioSciences

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Sumo1 cDNA ORF Clone, Rat, C-Myc tag

Sumo1 cDNA ORF Clone, Rat, C-Myc tag

SPD-14242

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Description
Full length Clone DNA of Rat SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae) with C terminal Myc tag.
Target Information
Species Rat
Target Name SUMO-1
Gene Abbr. Sumo1
Gene ID 301442
Full Name small ubiquitin-like modifier 1
Introduction Small ubiquitin-related modifier 1, 2 and 3 (SUMO-1, -2 and -3) are members of the ubiquitin-like protein family. The covalent attachment of the SUMO-1, -2 or -3 (SUMOylation) to target proteins is analogous to ubiquitination. This post-translational modification is a reversible, multi-step process that is initiated by cleaving a precursor protein to a mature protein. Mature SUMO-1, -2 or -3 is then linked to the activating enzyme E1, conjugated to E2 and in conjunction with E3, SUMO-1, -2 or -3 is ligated to the target protein. Ubiquitin and the individual SUMO family members are all targeted to different proteins with diverse biological functions. Ubiquitin predominantly regulates degradation of its target. In contrast, SUMO-1 is conjugated to RanGAP, PML, p53 and IκB-α to regulate nuclear trafficking, formation of subnuclear structures, regulation of transcriptional activity and protein stability. SUMO-2/-3 forms poly-(SUMO) chains, is conjugated to topoisomerase II and APP, regulates chromosomal segregation and cellular responses to environmental stress, and plays a role in the progression of Alzheimer disease.
Product Details
Description Full length Clone DNA of Rat SMT3 suppressor of mif two 3 homolog 1 (S. cerevisiae) with C terminal Myc tag.
NCBI Ref Seq NM_001009672.1
RefSeq ORF Size 306 bp
Vector pCMV3-C-Myc
Promoter Enhanced CMV promoter
Tag Sequence Myc Tag Sequence: GAGCAGAAACTCATCTCAGAAGAGGATCTG
Quality Control The plasmid is confirmed by full-length sequencing.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.
Usage
Sequencing Primers T7 (TAATACGACTCACTATAGGG); BGH (TAGAAGGCACAGTCGAGG);
Antibiotic in E.coli Kanamycin
Antibiotic in Mammalian cell Hygromycin
Application Stable or Transient mammalian expression
Shipping Each tube contains lyophilized plasmid.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.

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