SMURF2 cDNA ORF Clone, Human, C-FLAG tag - CD BioSciences

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SMURF2 cDNA ORF Clone, Human, C-FLAG tag

SMURF2 cDNA ORF Clone, Human, C-FLAG tag

SPD-13936

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Description
Full length Clone DNA of Human SMAD specific E3 ubiquitin protein ligase 2 with C terminal Flag tag.
Target Information
Species Human
Target Name SMURF
Gene Abbr. SMURF2
Gene ID 64750
Full Name SMAD specific E3 ubiquitin protein ligase 2
Introduction Smad ubiquitin regulatory factor 2 (Smurf2) is a HECT domain E3 ubiquitin ligase. It was initially identified as an inhibitor of TGF-β/BMP signaling by targeting R-Smads and TGF type I receptor for ubiquitination and degradation. Subsequent studies have revealed its role in neuronal and planar cell polarity, as well as in the senescence response and suppression of tumorigenesis. Smurf2 has a broad range of substrates including RUNX2, AMSH, Rap1B, and RNF11.Smurf2 is widely expressed in various tissues. The C2 domain of Smurf2 inhibits its catalytic activity by interacting with the HECT domain. Research studies have shown that Smurf2 functions as a tumor suppressor by maintaining genomic stability through targeting RNF20.
Product Details
Description Full length Clone DNA of Human SMAD specific E3 ubiquitin protein ligase 2 with C terminal Flag tag.
NCBI Ref Seq NM_022739.3
RefSeq ORF Size 2247 bp
Sequence Information Identical with the Gene Bank Ref. ID sequence.
Vector pCMV3-C-FLAG
Promoter Enhanced CMV promoter
Tag Sequence FLAG Tag Sequence: GATTACAAGGATGACGACGATAAG
Restriction Sites KpnI + XbaI (6kb + 2.29kb)
Quality Control The plasmid is confirmed by full-length sequencing.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.
Usage
Sequencing Primers T7 (TAATACGACTCACTATAGGG); BGH (TAGAAGGCACAGTCGAGG);
Antibiotic in E.coli Kanamycin
Antibiotic in Mammalian cell Hygromycin
Application Stable or Transient mammalian expression
Shipping Each tube contains lyophilized plasmid.
Storage The lyophilized plasmid can be stored at ambient temperature for three months.

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