Recombinant Rat Amphiregulin (Areg), partial, Yeast - CD BioSciences

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Recombinant Rat Amphiregulin (Areg), partial, Yeast

Recombinant Rat Amphiregulin (Areg), partial, Yeast

SPP-00178

Size Price
1 mg Online Inquiry
500 μg Online Inquiry
200 μg Online Inquiry
50 μg Online Inquiry
Target Information
Species Rattus norvegicus
Target Name Amphiregulin
UniProt No. P24338
Subcellular Location Membrane, Single-pass membrane protein
Gene Abbr. Areg
Full Name amphiregulin
Introduction Amphiregulin (AR) is a member of the EGF family of cytokines which is comprised of at least ten proteins including EGF, TGF-alpha, HB-EGF, and the various heregulins. All of these cytokines are synthesized as transmembrane precursors and are characterized by the presence of one or several EGF structural units in their extracellular domain. The soluble forms of these cytokines are released by proteolytic cleavage. Amphiregulin was originally isolated from the conditioned media of a PMA-treated MCF-7 human breast carcinoma cell line. The AR cDNA encodes a 252 amino acid (aa) residue transmembrane precursor. Multiple forms of native AR containing either 78 or 84 aa residues and both N- and O-linked oligosaccharides have been found. Amphiregulin mRNA expression can be detected in numerous carcinoma cell lines and the epithelial cells of various human tissues including colon, stomach, breast, ovary, kidney, etc.

Human AR stimulates the proliferation of various human and mouse keratinocytes, mammary epithelial cells and some fibroblasts. AR is also a growth inhibitor for various tumor cell lines. In certain colon carcinoma cell lines, AR has been shown to be an autocrine growth factor. Amphiregulin can bind to the EGF receptor. It has been suggested that in certain cell types, AR bioactivity may be mediated through the EGF receptor. The 98 aa residue long form of recombinant amphiregulin has shown to be approximately 5 - 10 fold more active than the 78 aa residue form of recombinant AR in an in vitro proliferation assay using Balb/3T3 fibroblasts.
Product Details
Product Type Recombinant Protein
Buffer before Lyophilization If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order.
Storage & Handling
Storage Temp. Store at -20 °C upon receipt unless otherwise instructed.
Handling Aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

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