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RCHY1 Antibody
SPA-09538
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | RCHY1 |
| Gene Abbr. | RCHY1 |
| Gene ID | 25898 |
| Full Name | ring finger and CHY zinc finger domain containing 1 |
| Alias | ARNIP, CHIMP, PIRH2, PRO1996, RNF199 |
| Introduction | RING finger and CHY1 zinc finger domain-containing protein 1 (RCHY1) is a newly identified RING-H2-type protein-ubiquitin E3 ligase that is expressed as multiple isoforms generated through alternative splicing of mRNA transcripts. Notably, RCHY1 is a p53-regulated gene and multiple studies have shown that RCHY1 directly binds p53 to promote its ubiquitin-dependent proteasomal degradation, ultimately leading to repression of p53 growth suppressive transcriptional activity. RCHY1 is also likely to promote deregulated cell proliferation by targeting the CDK inhibitor, p27 Kip1, for proteasomal degradation. In agreement with its growth promoting activity, RCHY1 overexpression has been linked to cancer of the lung and prostate. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic peptide corresponding to residues near the carboxy terminus of human RCHY1 protein isoform1. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human RCHY1 protein isoform1. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 29 |
| Reactivity | Human, Mouse |
| Sensitivity | Endogenous |
| Specificity | Detects endogenous levels of total RCHY1 protein. This antibody does not detect RCHY1 dimers. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.