PKA C-α Antibody - CD BioSciences

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PKA C-α Antibody

PKA C-α Antibody

SPA-08935

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100 µL Online Inquiry
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Target Information
Target Name PKA
Gene Abbr. PRKACA
Gene ID 5566
Full Name protein kinase cAMP-activated catalytic subunit alpha
Alias PKACA, PPNAD4
Introduction The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation. Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer. In this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (C-α, C-β, and C-γ) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. The two R families exist in two isoforms, α and β (RI-α, RI-β, RII-α, and RII-β). Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue. Substrates that present this consensus sequence and have been shown to be phosphorylated by PKA are Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3α Ser21 and GSK-3β Ser9). In addition, combined knock-down of PKA C-α and -β blocks cAMP-mediated phosphorylation of Raf (Ser43 and Ser259). Autophosphorylation and phosphorylation by PDK-1 are two known mechanisms responsible for phosphorylation of the C subunit at Thr197.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Immunogen Synthetic peptide corresponding to the carboxy terminal sequence of human PKA C-α.
Source/Purification Antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminal sequence of human PKA C-α. Antibodies are purified by protein A and peptide affinity chromatography.
Usage
Application WB, IP, IF, FC
Dilutions Western Blot (1:1000); Immunoprecipitation (1:50)
MW(KDa) 42
Reactivity Human, Mouse, Rat
Sensitivity Endogenous
Specificity It detects endogenous levels of total PKA C-α.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.