Phospho-YAP (Ser109) Antibody - CD BioSciences

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Phospho-YAP (Ser109) Antibody

Phospho-YAP (Ser109) Antibody

SPA-11872

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Target Information
Target Name YAP
Gene Abbr. YAP1
Gene ID 10413
Full Name Yes1 associated transcriptional regulator
Alias COB1, YAP, YAP2, YAP65, YKI
Introduction YAP (Yes-associated protein, YAP65) was first identified based on its ability to associate with the SH3 domain of Yes. It also binds to other SH3 domain-containing proteins such as Nck, Crk, Src, and Abl. In addition to the SH3 binding motif, YAP contains a PDZ interaction motif, a coiled-coil domain, and WW domains. While initial studies of YAP all pointed towards a role in anchoring and targeting to specific subcellular compartments, subsequent studies showed that YAP is a transcriptional co-activator by virtue of its WW domain interacting with the PY motif (PPxY) of the transcription factor PEBP2 and other transcription factors. In its capacity as a transcriptional co-activator, YAP is now widely recognized as a central mediator of the Hippo Pathway, which plays a fundamental and widely conserved role in regulating tissue growth and organ size. Phosphorylation at multiple sites (e.g., Ser109, Ser127) by LATS kinases promotes YAP translocation from the nucleus to the cytoplasm, where it is sequestered through association with 14-3-3 proteins. These LATS-driven phosphorylation events serve to prime YAP for subsequent phosphorylation by CK1δ/ε in an adjacent phosphodegron, triggering proteasomal degradation of YAP.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. E5I9G
Immunogen Synthetic peptide corresponding to residues surrounding Ser109 of human YAP protein.
Source/Purification Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser109 of human YAP protein.
Usage
Application WB, IP
Dilutions Western Blot (1:1000); Immunoprecipitation (1:50)
MW(KDa) 65-78
Reactivity Human, Mouse, Rat, Monkey
Sensitivity Endogenous
Specificity It recognizes endogenous levels of YAP protein only when phosphorylated at Ser109. Based on sequence similarity, the antibody may recognize TAZ protein when phosphorylated at Ser66.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.