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Phospho-PKA RII (Ser99) Antibody
SPA-08962
| Size | Price |
| 100 µL | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | PKA |
| Gene Abbr. | PRKAR2A |
| Gene ID | 5576 |
| Full Name | protein kinase cAMP-dependent type II regulatory subunit alpha |
| Alias | PKR2, PRKAR2 |
| Introduction | The second messenger cyclic AMP (cAMP) activates cAMP-dependent protein kinase (PKA or cAPK) in mammalian cells and controls many cellular mechanisms such as gene transcription, ion transport, and protein phosphorylation. Inactive PKA is a heterotetramer composed of a regulatory subunit (R) dimer and a catalytic subunit (C) dimer. In this inactive state, the pseudosubstrate sequences on the R subunits block the active sites on the C subunits. Three C subunit isoforms (C-α, C-β, and C-γ) and two families of regulatory subunits (RI and RII) with distinct cAMP binding properties have been identified. The two R families exist in two isoforms, α and β (RI-α, RI-β, RII-α, and RII-β). Upon binding of cAMP to the R subunits, the autoinhibitory contact is eased and active monomeric C subunits are released. PKA shares substrate specificity with Akt (PKB) and PKC, which are characterized by an arginine at position -3 relative to the phosphorylated serine or threonine residue. Substrates that present this consensus sequence and have been shown to be phosphorylated by PKA are Bad (Ser155), CREB (Ser133), and GSK-3 (GSK-3α Ser21 and GSK-3β Ser9). In addition, combined knock-down of PKA C-α and -β blocks cAMP-mediated phosphorylation of Raf (Ser43 and Ser259). Autophosphorylation and phosphorylation by PDK-1 are two known mechanisms responsible for phosphorylation of the C subunit at Thr197. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | SC54-04 |
| Isotype | IgG |
| Immunogen | Synthetic phospho-peptide surrounding S99 of PKAR2. |
| Usage | |
|---|---|
| Application | WB, IF, IHC, IP |
| Dilutions | Western Blot (1:100-1:2000); Immunofluorescence (1:10-1:500); Immunohistochemistry (1:10-1:500) |
| MW(KDa) | 49-55 |
| Reactivity | Human, Mouse, Rat, Porcine |
| Storage & Handling | |
|---|---|
| Storage Buffer | TBS (pH 7.4), 1% BSA, 40% Glycerol. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.