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Phospho-Phospholamban (Ser16) Antibody
SPA-08834
| Size | Price |
| 0.05 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Phospholamban |
| Gene Abbr. | PLN |
| Gene ID | 5350 |
| Full Name | phospholamban |
| Alias | CMD1P, CMH18, PLB |
| Introduction | Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1-25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (aa25-52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | Synthetic peptide corresponding to aa14-25, RA[pS]TIEMPQQAR-C of human cardiac phospholamban with a cysteine added at the C-terminal, conjugated to KLH. Species Sequence Homology: mouse, rat, rabbit, porcine and canine. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (0.5-2 µg/mL) |
| Reactivity | Human |
| Specificity | Recognizes bovine Phospho-lamban phosphorylated (Ser16) (5-25kD). Additional non-specific bands may be detected at 75 and 100kD in some lysates. |
| Storage & Handling | |
|---|---|
| Storage Buffer | 0.1 M Tris-Glycine (pH 7.4), 0.15 M NaCl and 30% Glycerol. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage. |
| Handling | Avoid freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.