Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody - CD BioSciences

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Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody

Phospho-MKK3 (Ser189)/MKK6 (Ser207) Antibody

SPA-07619

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Target Information
Target Name MKK3/MKK6
Gene Abbr. MAP2K3
Gene ID 5606
Full Name mitogen-activated protein kinase kinase 3
Alias MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2
Introduction MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase. MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines. Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr222 on MKK3 and Ser207 and Thr211 on MKK6.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. D8E9
Immunogen Synthetic phosphopeptide corresponding to residues surrounding Ser189 of human MKK3 protein and Ser207 of human MKK6 protein.
Source/Purification Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser189 of human MKK3 protein and Ser207 of human MKK6 protein.
Usage
Application WB, IP, IF
Dilutions Western Blot (1:1000); Immunoprecipitation (1:100)
MW(KDa) 40 (P-MKK3), 41 (P-MKK6)
Reactivity Human, Mouse, Rat, Monkey
Sensitivity Endogenous
Specificity It recognizes endogenous levels of MKK3 and MKK6 proteins only when phosphorylated at Ser189 (MKK3) or Ser207 (MKK6).
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.