Phospho-MEK1 (Ser218/Ser222) Antibody - CD BioSciences

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Phospho-MEK1 (Ser218/Ser222) Antibody

Phospho-MEK1 (Ser218/Ser222) Antibody

SPA-07398

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Target Information
Target Name MEK1
Gene Abbr. MAP2K1
Gene ID 5604
Full Name mitogen-activated protein kinase kinase 1
Alias CFC3, MAPKK1, MEK1, MEL, MKK1
Introduction MEK1 and MEK2, also called MAPK or Erk kinases, are dual-specificity protein kinases that function in a mitogen activated protein kinase cascade controlling cell growth and differentiation. Activation of MEK1 and MEK2 occurs through phosphorylation of two serine residues at positions 217 and 221, located in the activation loop of subdomain VIII, by Raf-like molecules. MEK1/2 is activated by a wide variety of growth factors and cytokines and also by membrane depolarization and calcium influx. Constitutively active forms of MEK1/2 are sufficient for the transformation of NIH/3T3 cells or the differentiation of PC-12 cells. MEK activates p44 and p42 MAP kinase by phosphorylating both threonine and tyrosine residues at sites located within the activation loop of kinase subdomain VIII.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser218/222 conjugated to KLH.
Usage
Application WB
Dilutions Western Blot (1:1000)
MW(KDa) 45
Reactivity Human, Mouse, Human, Rat, Bovine, Canine, Chicken, Primate, Xenopus, Zebrafish
Specificity Specific for the ~45k MEK 1/2 protein phosphorylated at Ser218/222 in Western Blots. Immunolabeling is blocked by the phosphopeptide used as the antigen but not by the corresponding dephosphopeptide.
Storage & Handling
Storage Buffer 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA and 50% Glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Avoid freeze-thaw cycles.

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