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Phospho-MARCKS (Ser159/163) Antibody
SPA-07227
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | MARCKS |
| Gene Abbr. | MARCKS |
| Gene ID | 4082 |
| Full Name | myristoylated alanine rich protein kinase C substrate |
| Alias | 80K-L, MACS, PKCSL, PRKCSL |
| Introduction | Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis. PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS. Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D13D2 |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Ser159 and Ser163 of human MARCKS protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser159 and Ser163 of human MARCKS protein. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:100) |
| MW(KDa) | 75 (rodent), 80 (human) |
| Reactivity | Human, Mouse, Rat |
| Sensitivity | Endogenous |
| Specificity | This antibody recognizes endogenous levels of MARCKS protein when phosphorylated at Ser159 and Ser163 (human residues). |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.