Phospho-LRP6 (Ser1490) Antibody - CD BioSciences

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Phospho-LRP6 (Ser1490) Antibody

Phospho-LRP6 (Ser1490) Antibody

SPA-07041

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Target Information
Target Name LRP6
Gene Abbr. LRP5
Gene ID 4041
Full Name LDL receptor related protein 5
Alias BMND1, EVR1, EVR4, HBM, LR3, LRP-5, LRP-7
Introduction LRP5 and LRP6 are single-pass transmembrane proteins belonging to the low-density lipoprotein receptor (LDLR)-related protein family. Unlike other members of the LDLR family, LRP5 and LRP6 have four EGF and three LDLR repeats in the extracellular domain, and proline-rich motifs in the cytoplasmic domain. They function as co-receptors for Wnt and are required for the canonical Wnt/β-catenin signaling pathway. LRP5 and LRP6 are highly homologous and have redundant roles during development. The activity of LRP5 and LRP6 can be inhibited by the binding of some members of the Dickkopf (DKK) family of proteins. Upon stimulation with Wnt, LRP6 is phosphorylated at multiple sites including Thr1479, Ser1490, and Thr1493 by kinases such as GSK-3 and CK1. Phosphorylated LRP6 recruits axin to the membrane and presumably activates β-catenin signaling.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Immunogen Synthetic phosphopeptide corresponding to residues surrounding Ser1490 of human LRP6.
Source/Purification Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser1490 of human LRP6. Antibodies are purified by protein A and peptide affinity chromatography.
Usage
Application WB, IP
Dilutions Western Blot (1:1000); Immunoprecipitation (1:200)
MW(KDa) 180, 210
Reactivity Human
Sensitivity Endogenous
Specificity It detects endogenous levels of LRP6 protein when phosphorylated at serine 1490. It is expected, although not yet confirmed, to also detect endogenous levels of LRP5 protein when phosphorylated at serine 1493 (corresponding to serine 1490 in LRP6).
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.