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Phospho-LDHA (Tyr10) Antibody
SPA-06871
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Lactate Dehydrogenase |
| Gene Abbr. | LDHA |
| Gene ID | 3939 |
| Full Name | lactate dehydrogenase A |
| Alias | GSD11, HEL-S-133P, LDHM, PIG19 |
| Introduction | Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites. LDHA expression is induced under hypoxic conditions. During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain.Recent studies indicate that LDHA is phosphorylated at Tyr10 and Tyr83 by the receptor tyrosine kinase FGFR1. Phosphorylation at Tyr10 up-regulates the activity of LDHA and is found in a variety of human cancer cells. Results further suggest that tyrosine phosphorylation regulates the NADH/NAD+ redox homeostasis and thus promotes the Warburg effect and tumor cell growth. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic peptide corresponding to residues surrounding Tyr10 of human LDHA protein. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Tyr10 of human LDHA protein. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 37 |
| Reactivity | Human, Mouse, Rat |
| Sensitivity | Endogenous |
| Specificity | This antibody recognizes endogenous levels of LDHA protein only when phosphorylated at Tyr10. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.