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Phospho-LAT (Tyr255) Antibody
SPA-06913
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | LAT |
| Gene Abbr. | LAT |
| Gene ID | 27040 |
| Full Name | linker for activation of T cells |
| Alias | IMD52, LAT1, pp36 |
| Introduction | LAT, a transmembrane adaptor protein expressed in T, NK and mast cells, is an important mediator for T cell receptor (TCR) signaling. Upon TCR engagement, activated Zap-70 phosphorylates LAT at multiple conserved tyrosine residues within SH2 binding motifs, exposing these motifs as the docking sites for downstream signaling targets. The phosphorylation of LAT at Tyr171 and Tyr191 enables the binding of Grb2, Gads/SLP-76, PLCγ1 and PI3 kinase through their SH2 domain and translocates them to the membrane. This process eventually leads to activation of the corresponding signaling pathways.Research studies have shown that LAT is also phosphorylated at Tyr225 by Zap-70 and Syk following activation of the TCR, which facilitates its binding to Grb2 and Vav and subsequent downstream activation of ERK. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | E3K6B |
| Immunogen | Synthetic phosphopeptide corresponding to residues surrounding Tyr255 of human LAT protein, isoform 1. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr255 of human LAT protein, isoform 1. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:200) |
| MW(KDa) | 40 |
| Reactivity | Human, Mouse |
| Sensitivity | Endogenous |
| Specificity | This antibody recognizes endogenous levels of LAT protein only when phosphorylated at Tyr255. This antibody cross-reacts with a 70 kDa phospho-protein of unknown origin in some treated cell lines. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.