Phospho-IKKα/β (Ser176/180) Antibody - CD BioSciences

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Phospho-IKKα/β (Ser176/180) Antibody

Phospho-IKKα/β (Ser176/180) Antibody

SPA-05471

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Target Information
Target Name IKKα/β
Gene Abbr. IKBKA/IKBKB
Gene ID 1147
Full Name component of inhibitor of nuclear factor kappa B kinase complex
Alias IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16
Introduction The NF-κB/Rel transcription factors are present in the cytosol in an inactive state, complexed with the inhibitory IκB proteins. Most agents that activate NF-κB do so through a common pathway based on phosphorylation-induced, proteasome-mediated degradation of IκB. The key regulatory step in this pathway involves activation of a high molecular weight IκB kinase (IKK) complex whose catalysis is generally carried out by three tightly associated IKK subunits. IKKα and IKKβ serve as the catalytic subunits of the kinase and IKKγ serves as the regulatory subunit. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. 16A6
Immunogen Synthetic phosphopeptide corresponding to residues surrounding Ser176/180 of human IKKα.
Source/Purification Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser176/180 of human IKKα.
Usage
Application WB, IHC, FC
Dilutions Western Blot (1:1000)
MW(KDa) 85 (alpha), 87 (beta)
Reactivity Human, Mouse, Rat, Monkey
Sensitivity Endogenous
Specificity It detects IKKα only when phosphorylated at Ser176/180 and IKKβ only when phosphorylated at Ser177/181.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.