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Phospho-HNF1α (Ser247) Antibody
SPA-05205
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | HNF1α |
| Gene Abbr. | HNF1A |
| Gene ID | 6927 |
| Full Name | HNF1 homeobox A |
| Alias | HNF-1A, HNF1, HNF4A, IDDM20, LFB1 |
| Introduction | Hepatocyte nuclear factor 1α (HNF1α, also known as TCF1 or MODY3) is a transcription factor that plays a role in the tissue-specific regulation of liver gene expression. Research has shown that heterogeneous mutations of HNF1α are linked to maturity onset diabetes of the young (MODY). Recent studies indicate that increased concentrations of free fatty acids can reduce the expression of FoxA2/HNF3β and HNF1α in pancreatic β-cells and lead to their nuclear exclusion, resulting in symptoms of several metabolic syndromes.HNF1α is inhibited through Akt2-mediated phosphorylation at Ser247 and is a negative regulator of PPARγ gene transcription. Research studies have shown that inhibition of PPARγ is beneficial in steatosis-associated liver cancer in mouse models. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Immunogen | Synthetic peptide corresponding to residues surrounding Ser247 of human HNF1α protein. |
| Source/Purification | Antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser247 of human HNF1α protein. Antibodies are purified by protein A and peptide affinity chromatography. |
| Usage | |
|---|---|
| Application | WB |
| Dilutions | Western Blot (1:1000) |
| MW(KDa) | 81 |
| Reactivity | Human |
| Sensitivity | Endogenous |
| Specificity | It recognizes endogenous levels of HNF1α protein only when phosphorylated at Ser247. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol. |
| Preservative | No Preservative |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.