Phospho-GSK-3α/β (Ser21/9) Antibody - CD BioSciences

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Phospho-GSK-3α/β (Ser21/9) Antibody

Phospho-GSK-3α/β (Ser21/9) Antibody

SPA-04883

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25 µg Online Inquiry
100 µg Online Inquiry
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Target Information
Target Name GSK-3
Gene Abbr. GSK3A
Gene ID 2931
Full Name glycogen synthase kinase 3 alpha
Introduction Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin. GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β. GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development. GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. D17D2
Immunogen Synthetic phosphopeptide corresponding to residues surrounding Ser21 of human GSK-3α protein.
Source/Purification Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser21 of human GSK-3α protein.
Usage
Application WB, IP
Dilutions Western Blot (1:1000); Immunoprecipitation (1:50)
MW(KDa) 46 (beta), 51 (alpha)
Reactivity Human, Mouse, Rat, Hamster, Monkey
Sensitivity Endogenous
Specificity It recognizes endogenous levels of GSK-3α and GSK-3β proteins only when phosphorylated at Ser21 or Ser9, respectively.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.