Phospho-EGF Receptor (Tyr1173) Antibody - CD BioSciences

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Phospho-EGF Receptor (Tyr1173) Antibody

Phospho-EGF Receptor (Tyr1173) Antibody

SPA-03318

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Target Information
Target Name EGFR
Gene Abbr. EGFR
Gene ID 1956
Full Name epidermal growth factor receptor
Alias ERBB, ERBB1, HER1, NISBD2, PIG61
Introduction The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation. Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins. c-Src is involved in phosphorylation of EGFR at Tyr845. The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling. Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation. The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068. A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation. Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. RM269
Isotype IgG
Immunogen A phospho-peptide corresponding to Human EGFR [p Tyr1173].
Usage
Application WB, IHC
Dilutions Immunohistochemistry (1:500-1:1000)
MW(KDa) 175
Reactivity Human
Specificity This antibody reacts to human EGF Receptor only when phosphorylated at Tyr1173. There is no cross-reactivity to EGFR without phosphorylation at Tyr1173.
Storage & Handling
Storage Buffer 50% Glycerol/PBS with 1% BSA.
Preservative 0.09% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Avoid freeze-thaw cycles.

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