Phospho-EGF Receptor (Tyr1173) Antibody - CD BioSciences

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Phospho-EGF Receptor (Tyr1173) Antibody

Phospho-EGF Receptor (Tyr1173) Antibody

SPA-03315

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Target Information
Target Name EGFR
Gene Abbr. EGFR
Gene ID 1956
Full Name epidermal growth factor receptor
Alias ERBB, ERBB1, HER1, NISBD2, PIG61
Introduction The epidermal growth factor (EGF) receptor is a transmembrane tyrosine kinase that belongs to the HER/ErbB protein family. Ligand binding results in receptor dimerization, autophosphorylation, activation of downstream signaling, internalization, and lysosomal degradation. Phosphorylation of EGF receptor (EGFR) at Tyr845 in the kinase domain is implicated in stabilizing the activation loop, maintaining the active state enzyme, and providing a binding surface for substrate proteins. c-Src is involved in phosphorylation of EGFR at Tyr845. The SH2 domain of PLCγ binds at phospho-Tyr992, resulting in activation of PLCγ-mediated downstream signaling. Phosphorylation of EGFR at Tyr1045 creates a major docking site for the adaptor protein c-Cbl, leading to receptor ubiquitination and degradation following EGFR activation. The GRB2 adaptor protein binds activated EGFR at phospho-Tyr1068. A pair of phosphorylated EGFR residues (Tyr1148 and Tyr1173) provide a docking site for the Shc scaffold protein, with both sites involved in MAP kinase signaling activation. Phosphorylation of EGFR at specific serine and threonine residues attenuates EGFR kinase activity. EGFR carboxy-terminal residues Ser1046 and Ser1047 are phosphorylated by CaM kinase II; mutation of either of these serines results in upregulated EGFR tyrosine autophosphorylation.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. 53A5
Immunogen Synthetic phosphopeptide corresponding to residues surrounding Tyr1173 of human EGF receptor.
Source/Purification Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr1173 of human EGF receptor.
Usage
Application WB, IP, IHC
Dilutions Western Blot (1:1000); Immunoprecipitation (1:50)
MW(KDa) 175
Reactivity Human, Mouse, Rat
Sensitivity Endogenous
Specificity It detects endogenous EGF receptors only when phosphorylated at Tyr1173. This antibody may cross-react with other activated receptor tyrosine kinases.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

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