Phospho-eEF2K (Ser366) Antibody - CD BioSciences

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Phospho-eEF2K (Ser366) Antibody

Phospho-eEF2K (Ser366) Antibody

SPA-03235

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25 µg Online Inquiry
100 µg Online Inquiry
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Target Information
Target Name eEF2K
Gene Abbr. EEF2K
Gene ID 29904
Full Name eukaryotic elongation factor 2 kinase
Alias CaMKIII, HSU93850, eEF-2K
Introduction Eukaryotic elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation. eEF2k is normally dependent on Ca2+ ions and calmodulin. It can be activated by PKA in response to elevated cAMP levels, which are generally increased in stress- or starvation-related conditions. eEF2k can also be regulated in response to a wide range of stimuli that promote cell growth and protein synthesis. This involves the phosphorylation of eEF2k by p90RSK and p70 S6 kinase at Ser366 or by SAPK4/p38delta at Ser359, leading to the inactivation of eEF2k, which facilitates the dephosphorylation of eEF2, and thus promotes translation.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Immunogen Synthetic phosphopeptide corresponding to residues surrounding Ser366 of human eEF2k.
Source/Purification Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser366 of human eEF2k. Antibodies are purified by protein A and peptide affinity chromatography.
Usage
Application WB, IP
Dilutions Western Blot (1:1000); Immunoprecipitation (1:100)
MW(KDa) 105
Reactivity Human, Rat, Monkey
Sensitivity Endogenous
Specificity It detects endogenous levels of eEF2k only when phosphorylated at Ser366.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.