Phospho-Cyclin B1 (Ser133) Antibody - CD BioSciences

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Phospho-Cyclin B1 (Ser133) Antibody

Phospho-Cyclin B1 (Ser133) Antibody

SPA-02604

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Target Information
Target Name Cyclin B1
Gene Abbr. CCNB1
Gene ID 890
Full Name cyclin B1
Alias CCNB
Introduction Cyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. This activation is a multi-step process that begins with the binding of the regulatory subunit, cyclin B1, to cdc2/cdk1 to form the mitosis-promoting factor (MPF). MPF remains in the inactive state until phosphorylation of cdc2/cdk1 at Thr161 by cdk activating kinase (CAK) and dephosphorylation of cdc2/cdk1 at Thr14/Tyr15 by cdc25C. Five cyclin B1 phosphorylation sites (Ser116, 126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis. While MPF itself can phosphorylate Ser126 and Ser128, polo-like kinase 1 (PLK1) phosphorylates cyclin B1 preferentially at Ser133 and possibly at Ser147. At the end of mitosis, cyclin B1 is targeted for degradation by the anaphase-promoting complex (APC), allowing for cell cycle progression. Research studies have shown that cyclin B1 is overexpressed in breast, prostate, and non-small cell lung cancers.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. 9E3
Immunogen Synthetic phosphopeptide corresponding to residues surrounding Ser133 of human cyclin B1.
Source/Purification Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser133 of human cyclin B1.
Usage
Application WB, IP
Dilutions Western Blot (1:1000); Immunoprecipitation (1:100)
MW(KDa) 55
Reactivity Human
Sensitivity Endogenous
Specificity It detects endogenous levels of cyclin B1 protein only when phosphorylated at Ser133.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.