Phospho-cPLA2 (Ser505) Antibody - CD BioSciences

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Phospho-cPLA2 (Ser505) Antibody

Phospho-cPLA2 (Ser505) Antibody

SPA-02323

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25 µg Online Inquiry
100 µg Online Inquiry
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Target Information
Target Name cPLA2
Gene Abbr. PLA2G4A
Gene ID 5321
Full Name phospholipase A2 group IVA
Alias GURDP, PLA2G4, cPLA2, cPLA2-alpha
Introduction Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and release arachidonic acid. cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity. Calcium binding to the amino-terminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane. Phosphorylation of cPLA2 by MAPK (p42/44 and p38) at Ser505 and Ser727 stimulates its catalytic activity.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Immunogen Synthetic phosphopeptide corresponding to residues surrounding Ser505 of human cPLA2.
Source/Purification Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser505 of human cPLA2. Antibodies are purified by protein A and peptide affinity chromatography.
Usage
Application WB, IP
Dilutions Western Blot (1:1000); Immunoprecipitation (1:50)
MW(KDa) 95
Reactivity Human, Mouse, Rat, Monkey
Sensitivity Endogenous
Specificity It detects endogenous levels of cPLA2 only when phosphorylated at serine 505. It does not cross-react with the secreted PLA2 (sPLA2) or cytosolic Ca2+ independent PLA2 (iPLA2).
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.