Phospho-Cdc2 (Thr161) Antibody - CD BioSciences

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Phospho-Cdc2 (Thr161) Antibody

Phospho-Cdc2 (Thr161) Antibody

SPA-02096

Size Price
25 µg Online Inquiry
100 µg Online Inquiry
More Options Online Inquiry
Target Information
Target Name Cdc2
Gene Abbr. CDC2
Gene ID 983
Full Name cyclin dependent kinase 1
Alias CDC2, CRCS10, MDPL, POLD
Introduction The entry of eukaryotic cells into mitosis is regulated by cdc2 kinase activation, a process controlled at several steps including cyclin binding and phosphorylation of cdc2 at Thr161. However, the critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of cdc2 at Thr14 and Tyr15. Phosphorylation at Thr14 and Tyr15, resulting in inhibition of cdc2, can be carried out by Wee1 and Myt1 protein kinases. The cdc25 phosphatase may be responsible for removal of phosphates at Thr14 and Tyr15 and subsequent activation of cdc2.cdc2 activation and association with cyclin A require phosphorylation at Thr161 by the CDK-activating kinase CAK, a complex of CDK7 and cyclin H.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Immunogen Synthetic phosphopeptide corresponding to residues surrounding Thr161 of human cdc2.
Source/Purification Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr161 of human cdc2. Antibodies are purified by protein A and peptide affinity chromatography.
Usage
Application WB
Dilutions Western Blot (1:1000)
MW(KDa) 34
Reactivity Human, Mouse, Rat, Monkey
Sensitivity Endogenous
Specificity It detects endogenous levels of cdc2 only when phosphorylated at threonine 161. The antibody cross-reacts with endogenous CDK2 phosphorylated at threonine 160.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.