Phospho-Catenin δ-1 (Tyr228) Antibody - CD BioSciences

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Phospho-Catenin δ-1 (Tyr228) Antibody

Phospho-Catenin δ-1 (Tyr228) Antibody

SPA-01466

Size Price
25 µg Online Inquiry
100 µg Online Inquiry
More Options Online Inquiry
Target Information
Target Name Catenin δ-1
Gene Abbr. CTNND1
Gene ID 1500
Full Name catenin delta 1
Alias BCDS2, CAS, CTNND, P120CAS, P120CTN
Introduction Catenin δ-1 (p120 catenin) has an amino-terminal coiled-coil domain followed by a regulatory domain containing multiple phosphorylation sites and a central Armadillo repeat domain of ten linked 42-amino acid repeats. The carboxy-terminal tail has no known function. Catenin δ-1 fulfills critical roles in the regulation of cell-cell adhesion as it regulates E-cadherin turnover at the cell surface to determine the level of E-cadherin available for cell-cell adhesion. Catenin δ-1 has both positive and negative effects on cadherin-mediated adhesion. Actin dynamics are also regulated by catenin δ-1, which modulates RhoA, Rac, and cdc42 proteins. Analogous to β-catenin, catenin δ-1 translocates to the nucleus, although its role at this location is unclear. Many studies show that catenin δ-1 is expressed irregularly or is absent in various types of tumor cells, suggesting that catenin δ-1 may function as a tumor suppressor.Catenin δ-1 is phosphorylated at multiple tyrosine sites along its sequence both in vivo and in vitro. High levels of catenin δ-1 phosphorylated at Tyr228 are commonly seen in several carcinoma cell lines. EGFR signaling induces catenin δ-1 phosphorylation at Tyr228, with the phosphorylated protein becoming localized at adherens junctions although phosphorylation is not essential in junction formation.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Immunogen Synthetic phosphopeptides corresponding to residues surrounding Tyr228 of human/mouse catenin δ-1.
Source/Purification Antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding Tyr228 of human/mouse catenin δ-1. Antibodies are purified by peptide affinity chromatography.
Usage
Application WB, IF
Dilutions Western Blot (1:1000)
MW(KDa) 95, 100
Reactivity Human
Sensitivity Endogenous
Specificity It detects endogenous levels of catenin δ-1 protein only when phosphorylated at Tyr228. The antibody might cross react with another overexpressed phospho-tyrosine protein.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.