Phospho-c-Raf (Ser642) Antibody - CD BioSciences

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Phospho-c-Raf (Ser642) Antibody

Phospho-c-Raf (Ser642) Antibody

SPA-01281

Size Price
0.1 mL Online Inquiry
More Options Online Inquiry
Target Information
Target Name c-Raf
Gene Abbr. RAF1
Gene ID 5894
Full Name Raf-1 proto-oncogene, serine/threonine kinase
Alias CMD1NN, CRAF, NS5, Raf-1, c-Raf
Introduction A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497, and Ser499. p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity. Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf. Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively. While A-Raf, B-Raf, and c-Raf are similar in sequence and function, differential regulation has been observed. Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428, and Thr439) and lacks a site equivalent to Tyr341 of c-Raf. Research studies have shown that the B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma. Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301, and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser642 conjugated to KLH.
Usage
Application WB
Dilutions Western Blot (1:1000)
MW(KDa) 75
Reactivity Human, Rat, Human, Mouse, Bovine, Canine, Chicken, Primate
Specificity Specific for the ~74k Raf-1 protein phosphorylated at Ser642.
Storage & Handling
Storage Buffer 10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA and 50% Glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Avoid freeze-thaw cycles.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.