Phospho-c-Raf (Ser259) Antibody - CD BioSciences

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Phospho-c-Raf (Ser259) Antibody

Phospho-c-Raf (Ser259) Antibody

SPA-01272

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Target Information
Target Name c-Raf
Gene Abbr. RAF1
Gene ID 5894
Full Name Raf-1 proto-oncogene, serine/threonine kinase
Alias CMD1NN, CRAF, NS5, Raf-1, c-Raf
Introduction A-Raf, B-Raf, and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway. Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497, and Ser499. p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338, and the Src family phosphorylates Tyr341 to induce c-Raf activity. Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf. Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively. While A-Raf, B-Raf, and c-Raf are similar in sequence and function, differential regulation has been observed. Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428, and Thr439) and lacks a site equivalent to Tyr341 of c-Raf. Research studies have shown that the B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma. Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301, and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen Synthetic phospho-peptide surrounding amino acid Ser259 of human c-Raf.
Usage
Application WB, IHC
MW(KDa) 75
Reactivity Human, Mouse, Rat
Storage & Handling
Storage Buffer PBS (pH 7.2), 1% BSA, 50% Glycerol.
Preservative 0.02% Thimerosal
Storage Temp. Store at -20 °C.
Handling Avoid freeze-thaw cycles.

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