Phospho-c-Abl (Tyr412) Antibody - CD BioSciences

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Phospho-c-Abl (Tyr412) Antibody

Phospho-c-Abl (Tyr412) Antibody

SPA-01178

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Target Information
Target Name c-Abl
Gene Abbr. ABL1
Gene ID 25
Full Name ABL proto-oncogene 1, non-receptor tyrosine kinase
Alias ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl
Introduction The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion, and stress responses. c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation; PDGF stimulation; and binding to c-Jun, Nck, and RFX1. The in vivo mechanism for regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation at Tyr245 is involved in the activation of c-Abl kinase. In addition, phosphorylation at Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. 247C7
Immunogen Synthetic phosphopeptide corresponding to residues surrounding Tyr412 of human c-Abl.
Source/Purification Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr412 of human c-Abl.
Usage
Application WB
Dilutions Western Blot (1:1000)
MW(KDa) 135 (c-Abl), 210 (Bcr-Abl)
Reactivity Human
Sensitivity Endogenous
Specificity It detects endogenous Abl proteins only when phosphorylated at tyrosine 412. The antibody does not cross-react with other phosphorylated tyrosine kinases.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

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