Phospho-Btk (Tyr223) Antibody - CD BioSciences

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Phospho-Btk (Tyr223) Antibody

Phospho-Btk (Tyr223) Antibody

SPA-01154

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Target Information
Target Name Btk
Gene Abbr. BTK
Gene ID 695
Full Name Bruton tyrosine kinase
Alias AGMX1, AT, ATK, BPK, IGHD3
Introduction Bruton's tyrosine kinase (Btk) is a member of the Btk/Tec family of cytoplasmic tyrosine kinases. Like other Btk family members, it contains a pleckstrin homology (PH) domain and Src homology SH3 and SH2 domains. Btk plays an important role in B cell development. Activation of B cells by various ligands is accompanied by Btk membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane-localized Btk is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinases, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation. The activation of Btk is negatively regulated by PKCβ through phosphorylation of Btk at Ser180, which results in reduced membrane recruitment, transphosphorylation, and subsequent activation. The PKC inhibitory signal is likely to be a key determinant of the B cell receptor signaling threshold to maintain optimal Btk activity.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. D1D2Z
Immunogen Synthetic phosphopeptide corresponding to residues surrounding Tyr223 of human Btk protein.
Source/Purification Antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr223 of human Btk protein.
Usage
Application WB, IP
Dilutions Western Blot (1:1000); Immunoprecipitation (1:100)
MW(KDa) 78
Reactivity Human, Mouse
Sensitivity Endogenous
Specificity This antibody recognizes endogenous levels of Btk protein only when phosphorylated at Tyr223. The antibody detects a 26 kDa protein of unknown identity that is not sensitive to treatment with anti-IgM or Ibrutinib.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

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