Phospho-Bad (Ser112) Antibody - CD BioSciences

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Phospho-Bad (Ser112) Antibody

Phospho-Bad (Ser112) Antibody

SPA-00838

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Target Information
Target Name BAD
Gene Abbr. BAD
Gene ID 572
Full Name BCL2 associated agonist of cell death
Alias BBC2, BCL2L8
Introduction Bad is a proapoptotic member of the Bcl-2 family that promotes cell death by displacing Bax from binding to Bcl-2 and Bcl-xL. Survival factors, such as IL-3, inhibit the apoptotic activity of Bad by activating intracellular signaling pathways that result in the phosphorylation of Bad at Ser112 and Ser136. Phosphorylation at these sites promotes binding of Bad to 14-3-3 proteins to prevent an association between Bad with Bcl-2 and Bcl-xL. Akt phosphorylates Bad at Ser136 to promote cell survival. Bad is phosphorylated at Ser112 both in vivo and in vitro by p90RSK and mitochondria-anchored PKA. Phosphorylation at Ser155 in the BH3 domain by PKA plays a critical role in blocking the dimerization of Bad and Bcl-xL.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen Synthesized phosphopeptide derived from human BAD around the phosphorylation site of serine 112 (H-S-SP-Y-P).
Usage
Application WB, IHC
Dilutions Western Blot (1:500-1:1000); Immunohistochemistry (1:50-1:100)
MW(KDa) 23
Reactivity Human, Mouse, Primate
Specificity BAD (specific to Phospho-Ser112) detects endogenous levels of BAD only when phosphorylated at serine 112.
Storage & Handling
Storage Buffer PBS (without Mg2+, Ca2+) (pH 7.4), 150 mM NaCl, 50% Glycerol.
Preservative 0.02% Sodium Azide
Storage Temp. Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage.
Handling Avoid freeze-thaw cycles.

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