Phospho-ATR (Thr1989) Antibody - CD BioSciences

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Phospho-ATR (Thr1989) Antibody

Phospho-ATR (Thr1989) Antibody

SPA-00780

Size Price
25 µL Online Inquiry
0.1 mL Online Inquiry
More Options Online Inquiry
Target Information
Target Name ATR
Gene Abbr. ATR
Gene ID 545
Full Name ATR serine/threonine kinase
Alias FCTCS, FRP1, MEC1, SCKL, SCKL1
Introduction Ataxia telangiectasia mutated kinase (ATM) and ataxia telangiectasia and Rad3-related kinase (ATR) are PI3 kinase-related kinase (PIKK) family members that phosphorylate multiple substrates on serine or threonine residues that are followed by a glutamine in response to DNA damage or replication blocks. Despite the essential role of ATR in cell cycle signaling and DNA repair processes, little is known about its activation. ATR was long thought to exist in a constitutively active state in cells, with DNA damage-induced signaling occurring via recruitment of ATR to single stranded DNA and sites of replication stress. Phosphorylation of ATR at serine 428 in response to UV-induced DNA damage has been suggested as a means of activating ATR. Recent work has shown autophosphorylation of ATR at threonine 1989. Like ATM Ser1981, phosphorylation of ATR Thr1989 occurs in response to DNA damage, indicating that phosphorylation at this site is important in ATR-mediated signaling.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. D5K8W
Immunogen Synthetic peptide corresponding to residues surrounding Thr1989 of human ATR protein.
Source/Purification Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr1989 of human ATR protein.
Usage
Application WB
Dilutions Western Blot (1:1000)
MW(KDa) 300
Reactivity Human
Sensitivity Endogenous
Specificity It recognizes endogenous levels of ATR protein only when phosphorylated at Thr1989.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

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