Phospho-ATF-2 (Thr71) Antibody - CD BioSciences


Phospho-ATF-2 (Thr71) Antibody

Phospho-ATF-2 (Thr71) Antibody


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0.025 mg Online Inquiry
0.1 mg Online Inquiry
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Target Information
Target Name ATF-2
Gene Abbr. ATF2
Gene ID 1386
Full Name activating transcription factor 2
Alias CRE-BP1, CREB-2, CREB2, HB16, TREB7
Introduction The transcription factor ATF-2 (also called CRE-BP1) binds to both AP-1 and CRE DNA response elements and is a member of the ATF/CREB family of leucine zipper proteins. ATF-2 interacts with a variety of viral oncoproteins and cellular tumor suppressors and is a target of the SAPK/JNK and p38 MAP kinase signaling pathways. Various forms of cellular stress, including genotoxic agents, inflammatory cytokines, and UV irradiation, stimulate the transcriptional activity of ATF-2. Cellular stress activates ATF-2 by phosphorylation of Thr69 and Thr71. Both SAPK and p38 MAPK have been shown to phosphorylate ATF-2 at these sites in vitro and in cells transfected with ATF-2. Mutations of these sites result in the loss of stress-induced transcription by ATF-2. In addition, mutations at these sites reduce the ability of E1A and Rb to stimulate gene expression via ATF-2.ATF-7 is another member of the ATF/CREB family of leucine zipper proteins. Similarly, Thr51 and Thr53 (corresponding to Thr69 and Thr71 of ATF-2, respectively) can be phosphorylated under different conditions.
Product Details
Host Mouse
Clonality Monoclonal
Clone No. 103C411.2
Isotype IgG1
Immunogen This antibody was generated by immunizing mice with a synthetic peptide containing phosphorylated threonine at position 71 of human ATF-2.
Application WB, FC
Dilutions Western Blot (2 µg/mL); Flow Cytometry (1:10-1:1000)
MW(KDa) 65, 75
Reactivity Human
Specificity It is specific for phospho (Thr71) ATF2. It cannot be used to test for non-phosphorylated ATF2.
Storage & Handling
Storage Buffer PBS containing 0.05% BSA.
Preservative 0.05% Sodium Azide
Storage Temp. Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage.
Handling Avoid freeze-thaw cycles.

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