Phospho-AS160 (Ser588) Antibody - CD BioSciences

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Phospho-AS160 (Ser588) Antibody

Phospho-AS160 (Ser588) Antibody

SPA-00570

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Target Information
Target Name AS160
Gene Abbr. TBC1D4
Gene ID 9882
Full Name TBC1 domain family member 4
Alias AS160, NIDDM5
Introduction Insulin is a major hormone controlling critical energy functions, such as glucose and lipid metabolism. Insulin binds to and activates the insulin receptor (IR) tyrosine kinase, which phosphorylates and recruits adaptor proteins. The signaling pathway initiated by insulin and its receptor stimulates glucose uptake in muscle cells and adipocytes through translocation of the Glut4 glucose transporter from the cytoplasm to the plasma membrane. A 160 kDa substrate of the Akt Ser/Thr kinase (AS160, TBC1D4) is a Rab GTPase-activating protein that regulates insulin-stimulated Glut4 trafficking. AS160 is expressed in many tissues including brain, kidney, liver, and brown and white fat. Multiple Akt phosphorylation sites have been identified on AS160 in vivo, with five sites (Ser318, Ser570, Ser588, Thr642, and Thr751) showing increased phosphorylation following insulin treatment. Studies using recombinant AS160 demonstrate that insulin-stimulated phosphorylation of AS160 is a crucial step in Glut4 translocation and is reduced in some patients with type 2 diabetes. The interaction of 14-3-3 regulatory proteins with AS160 phosphorylated at Thr642 is a necessary step for Glut4 translocation. Phosphorylation of AS160 by AMPK is involved in the regulation of contraction-stimulated Glut4 translocation.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen A synthetic phosphorylated peptide around S588 of human TBC1D4 (NP_055647.2).
Usage
Application WB
Dilutions Western Blot (1:500-1:2000)
MW(KDa) 160
Reactivity Human, Mouse, Rat
Storage & Handling
Storage Buffer PBS with 50% glycerol, pH 7.3.
Preservative 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Avoid freeze-thaw cycles.

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