Phospho-4E-BP1 (Thr70) Antibody - CD BioSciences

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Phospho-4E-BP1 (Thr70) Antibody

Phospho-4E-BP1 (Thr70) Antibody

SPA-00102

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100 µg Online Inquiry
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Target Information
Target Name 4EBP
Gene Abbr. EIF4EBP1
Gene ID 1978
Full Name eukaryotic translation initiation factor 4E binding protein 1
Alias 4E-BP1, 4EBP1, BP-1, PHAS-I
Introduction Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation. Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity. Multiple 4E-BP1 residues are phosphorylated in vivo. While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70.
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Immunogen Synthetic phosphopeptide corresponding to the sequence of rat 4E-BP1.
Source/Purification Antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to the sequence of rat 4E-BP1. Antibodies are purified by protein A and peptide affinity chromatography.
Usage
Application WB, IP
Dilutions Western Blot (1:1000); Immunoprecipitation (1:50)
MW(KDa) 15-20
Reactivity Human, Mouse, Rat, Monkey
Sensitivity Endogenous
Specificity It detects endogenous levels of 4E-BP1 only when phosphorylated at threonine 70. This antibody does not detect 4E-BP1 phosphorylated at other sites.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA and 50% glycerol.
Preservative No Preservative
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.