PEDFR/PNPLA2/ATGL Antibody - CD BioSciences

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PEDFR/PNPLA2/ATGL Antibody

PEDFR/PNPLA2/ATGL Antibody

SPA-00728

Size Price
0.025 mL Online Inquiry
0.1 mL Online Inquiry
More Options Online Inquiry
Target Information
Target Name ATGL
Gene Abbr. PNPLA2
Gene ID 57104
Full Name patatin like phospholipase domain containing 2
Alias 1110001C14Rik, ATGL, FP17548, PEDF-R, TTS-2.2
Introduction ATGL (adipose triglyceride lipase) is the rate-limiting lipolytic enzyme which catalyzes initial step of triglyceride (TAG) hydrolysis in adipocytes as well as non-adipocyte lipid droplets. Among adipose lipases (ATGL, hormone-sensitive lipase/HSL and monoacylglycerol lipase), HSL is a diacylglycerol lipase, whereas ATGL prefers TAG substrates. CGI-58 coactivates ATGL's reactions and G0/G1 switch gene 2 exerts supressive effects on ATGL. Localized in lipid droplet and cell membrane as single-pass type II membrane protein, ATGL is mainly expressed in adipose tissue, however, has also been reported in cardiac/skeletal muscles, gastrointestinal tract, normal retina and retinoblastoma. ATGL is inhibited by BEL and is activated by ABHD5 as well as SERPINF1, and is induced during differentiation of primary preadipocytes to adipocytes with expression increasing from fetal to adult in RPE cells. Besides TAG hydrolysis, ATGL has acylglycerol transacylase activity also and can act coordinately with LIPE/HLS within the lipolytic cascade, regulate size of adiposome and implicate in adiposomes degradation. ATGL implicates in energy homeostasis/response to starvation by enhancing TAGs hydrolysis and providing free fatty acids to be oxidized in situations of energy depletion. ATGL genetic variations have been linked with risk of NIDDM and neutral lipid storage disease with myopathy (NLSDM).
Product Details
Host Rabbit
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen A synthetic peptide within the C-terminal region (residues 400-500) of human ATGL. [Accession# NP_005171].
Usage
Application WB, IF
Dilutions Western Blot (1:1000); Immunofluorescence (1:1000-1:2000)
Reactivity Human, Mouse
Storage & Handling
Storage Buffer Tris-Glycine and 0.15 M NaCl.
Preservative 0.05% Sodium Azide
Storage Temp. Aliquot and store at -20 °C or -80 °C.
Handling Avoid freeze-thaw cycles.

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