Online Inquiry
PAR1/Thrombin Receptor Antibody
SPA-10730
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Thrombin Receptor |
| Gene Abbr. | F2R |
| Gene ID | 2149 |
| Full Name | coagulation factor II thrombin receptor |
| Alias | CF2R, HTR, PAR-1, PAR1, TR |
| Introduction | Thrombin, the main effector protease of coagulation cascade, is a most potent platelet activator and its actions on platelets are mediated by Thrombin Receptors (also called platelet protease-activated receptors, PARs), which are GPCR members of 7-transmembrane domain receptor superfamily. PARs subtypes includes: PAR-1, PAR-2, PAR-3, and PAR-4, and in addition to platelets, they are also located in smooth muscle cells, endothelial cells, and fibroblasts. PAR1 or thrombin receptor (F2R) is a prototypical GPCR activated by thrombin's lowest concentration compared to other PARs and after cleavage of the extreme N-terminal peptide, the exposed tethered ligand of PAR1 activates itself for participation in diverse physiological processes including coagulation, inflammation, and vascular homeostasis. PAR1 knockout in mouse leads to death of pups in midgestation due to impaired vascular development as well as severe bleeding, whereas endothelial cell-specific reintroduction of PAR1 can rescue the phenotype. PAR1 has emerged as a target for anti-platelet therapies which are useful for ACS (acute coronary syndromes), stable coronary disease, ischemic atherothrombotic stroke, and peripheral arterial disease. |
| Product Details | |
|---|---|
| Host | Goat |
| Clonality | Polyclonal |
| Clone No. | N/A |
| Isotype | IgG |
| Immunogen | E. coli-derived recombinant human PAR1. Arg27-Thr102, Ser375-Thr425. P25116. |
| Usage | |
|---|---|
| Application | WB, FC, IF |
| Dilutions | Western Blot (0.1 µg/mL); Flow Cytometry (2.5 µg/10^6 cells); Immunofluorescence (5-15 µg/mL) |
| Reactivity | Human |
| Specificity | Detects human PAR1 in direct ELISAs and Western Blots. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.