p300 Antibody - CD BioSciences

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p300 Antibody

p300 Antibody

SPA-08377

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Target Information
Target Name p300
Gene Abbr. EP300
Gene ID 2033
Full Name E1A binding protein p300
Alias KAT3B, MKHK2, RSTS2, p300
Introduction CBP (CREB-binding protein) and p300 are highly conserved and functionally related transcriptional co-activators that associate with transcriptional regulators and signaling molecules, integrating multiple signal transduction pathways with the transcriptional machinery. CBP/p300 also contain histone acetyltransferase (HAT) activity, allowing them to acetylate histones and other proteins. Phosphorylation of p300 at Ser89 by PKC represses its transciptional acitivity, and phosphorylation at the same site by AMPK disrupts the association of p300 with nuclear receptors. Ser1834 phosphorylation of p300 by Akt disrupts its association with C/EBPβ. Growth factors induce phosphorylation of CBP at Ser437, which is required for CBP recruitment to the transcription complex. CaM kinase IV phosphorylates CBP at Ser302, which is required for CBP-dependent transcriptional activation in the CNS. The role of acetylation of CBP/p300 is of particular interest. Acetylation of p300 at Lys1499 has been demonstrated to enhance its HAT activity and affect a wide variety of signaling events.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. D1M7C
Immunogen Synthetic peptide corresponding to residues surrounding Leu64 of human p300 protein.
Source/Purification Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu64 of human p300 protein.
Usage
Application WB, IP
Dilutions Western Blot (1:1000); Immunoprecipitation (1:100)
MW(KDa) 300
Reactivity Human, Mouse, Monkey
Sensitivity Endogenous
Specificity It recognizes endogenous levels of total p300 protein. This antibody does not cross-react with CBP protein.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.