Online Inquiry
p190-A RhoGAP Antibody
SPA-08325
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | p190-A RhoGAP |
| Gene Abbr. | ARHGAP35 |
| Gene ID | 2909 |
| Full Name | Rho GTPase activating protein 35 |
| Alias | GRF-1, GRLF1, P190-A, P190A, p190ARhoGAP |
| Introduction | Rho family GTPases are key regulators of diverse processes such as cytoskeletal organization, cell growth and differentiation, transcriptional regulation, and cell adhesion/motility. The activities of these proteins are controlled primarily through guanine nucleotide exchange factors (GEFs) that facilitate the exchange of GDP for GTP, promoting the active (GTP-bound) state, and GTPase activating proteins (GAPs) that promote GTP hydrolysis and the inactive (GDP-bound) state.The p190 RhoGAP proteins are widely expressed Rho family GAPs. p190-A has been characterized as a tumor suppressor, and research studies have shown that loss or rearrangement of the chromosomal region containing the gene for p190-A is linked to tumor development. p190-A binds the mitogen-inducible transcription factor TFII-I, sequestering it in the cytoplasm and inhibiting its activity. Phosphorylation of p190-A at Tyr308 reduces its affinity for TFII-I, relieving the inhibition. p190-A can also inhibit growth factor-induced gliomas in mice (6) and affect cleavage furrow formation and cytokinesis in cultured cells.Mice lacking p190-B RhoGAP show excessive Rho activation and a reduction in activation of the transcription factor CREB. Cells deficient in p190-B display defective adipogenesis. There is increasing evidence that p190 undergoes tyrosine phosphorylation, which activates its GAP domain. Levels of tyrosine phosphorylation are enhanced by Src overexpression. IGF-I treatment downregulates Rho through phosphorylation and activation of p190-B RhoGAP, thereby enhancing IGF signaling implicated in adipogenesis. |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | D8Q6C |
| Immunogen | Synthetic peptide corresponding to residues surrounding Asn585 of human p190-A RhoGAP protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asn585 of human p190-A RhoGAP protein. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 190 |
| Reactivity | Human, Mouse, Rat, Hamster, Monkey |
| Sensitivity | Endogenous |
| Specificity | This antibody recognizes endogenous levels of total p190-A RhoGAP protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.