Online Inquiry
Occludin Antibody
SPA-08242
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Occludin |
| Gene Abbr. | OCLN |
| Gene ID | 100506658 |
| Full Name | occludin |
| Alias | BLCPMG, PPP1R115, PTORCH1 |
| Introduction | Tight junctions, or zona occludens, form a continuous barrier to fluids across the epithelium and endothelium. They function in regulation of paracellular permeability and in the maintenance of cell polarity, blocking the movement of transmembrane proteins between the apical and the basolateral cell surfaces. Tight junctions are composed of claudin and occludin transmembrane proteins, which join the junctions to the cytoskeleton. Occludin is thought to be important in the assembly and maintenance of tight junctions. Differential phosphorylation of occludin at various residues may regulate its interaction with other tight junction proteins such as ZO-1. VEGF-induced phosphorylation of occludin regulates tight junction stability and vascular permeability. Expression of occludin as well as claudin1 is required for infection of liver cells by hepatitis C virus (HCV). |
| Product Details | |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Clone No. | E6B4R |
| Immunogen | Synthetic peptide corresponding to residues surrounding Val294 of human occludin protein. |
| Source/Purification | Antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val294 of human occludin protein. |
| Usage | |
|---|---|
| Application | WB, IP, IF, IHC |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 65 |
| Reactivity | Human |
| Sensitivity | Endogenous |
| Specificity | This antibody recognizes endogenous levels of total occludin protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.