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Notch4 Antibody
SPA-08169
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | Notch4 |
| Gene Abbr. | NOTCH4 |
| Gene ID | 4855 |
| Full Name | notch receptor 4 |
| Alias | INT3 |
| Introduction | Notch proteins (Notch1-4) are a family of transmembrane receptors that play important roles in development and the determination of cell fate. Mature Notch receptors are processed and assembled as heterodimeric proteins, with each dimer comprised of a large extracellular ligand-binding domain, a single-pass transmembrane domain, and a smaller cytoplasmic subunit (Notch intracellular domain, NICD). Binding of Notch receptors to ligands of the Delta-Serrate-Lag2 (DSL) family triggers heterodimer dissociation, exposing the receptors to proteolytic cleavages; these result in release of the NICD, which translocates to the nucleus and activates transcription of downstream target genes.Notch4 is primarily expressed in endothelial cells and is processed similarly to Notch1. The mouse Notch4 gene (also known as Int-3) is a frequent target in Mouse Mammary Tumour Virus (MMTV)-induced mammary tumors. The intracellular domain of Notch4 binds to Smad3 and inhibits TGF-β signaling. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | L5C5 |
| Immunogen | Recombinant protein consisting of the Ankyrin repeat domain of human Notch4. |
| Source/Purification | Antibody is produced by immunizing animals with a recombinant protein consisting of the Ankyrin repeat domain of human Notch4. |
| Usage | |
|---|---|
| Application | WB, IP |
| Dilutions | Western Blot (1:1000); Immunoprecipitation (1:50) |
| MW(KDa) | 80 (cleaved), 230 (full-length) |
| Reactivity | Human |
| Sensitivity | Transfected Only |
| Specificity | It detects levels of the transfected intracellular region of human Notch4 protein. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. |
| Preservative | Less than 0.02% Sodium Azide |
| Storage Temp. | Store at -20 °C. |
| Handling | Do not aliquot the antibody. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.