NEDD4 Antibody - CD BioSciences

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NEDD4 Antibody

NEDD4 Antibody

SPA-07888

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Target Information
Target Name NEDD4
Gene Abbr. NEDD4
Gene ID 4734
Full Name NEDD4 E3 ubiquitin protein ligase
Alias NEDD4-1, RPF1
Introduction Neural precursor expressed, developmentally down-regulated protein 4 (NEDD4) was originally identified as a gene that is highly expressed in the early mouse embryonic central nervous system. Subsequently, a family of NEDD4-like proteins have been defined that includes seven members in humans. NEDD4 and NEDD4-like (NEDD4L) proteins contain multiple functional domains including a calcium-dependent phospholipid and membrane binding domain (C2 domain), two to four protein binding domains (WW domains), and an E3 ubiquitin-protein ligase domain (HECT domain). NEDD4 and NEDD4L have been shown to downregulate both neuronal voltage-gated Na+ channels (NaVs) and epithelial Na+ channels (ENaCs) in response to increased intracellular Na+ concentrations. The WW domains of NEDD4 bind to PY motifs (amino acid sequence PPXY) found in multiple NaV and ENaC proteins; ubiquitination of these proteins is mediated by the HECT domain of NEDD4 and results in their internalization and removal from the plasma membrane. Research studies have shown that mutation of the PY motifs in ENaC proteins is associated with Liddle's syndrome, an autosomal dominant form of hypertension. In addition to targeting sodium channels, NEDD4L has also been shown to negatively regulate TGF-β signaling by targeting Smad2 for degradation. Mouse and human NEDD4 are rapidly cleaved by caspase proteins during apoptosis, although the significance of this cleavage is not clear.
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. D82F4
Immunogen Synthetic peptide corresponding to human NEDD4 protein.
Source/Purification Antibody is produced by immunizing animals with a synthetic peptide corresponding to human NEDD4 protein.
Usage
Application WB, IP
Dilutions Western Blot (1:1000); Immunoprecipitation (1:50)
MW(KDa) 115
Reactivity Human, Rat, Monkey
Sensitivity Endogenous
Specificity It detects endogenous levels of total NEDD4. The antibody does not cross react with NEDD4L.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.