NCoR1 Antibody - CD BioSciences

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NCoR1 Antibody

NCoR1 Antibody

SPA-07862

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Target Information
Target Name NCoR1
Gene Abbr. NCOR1
Gene ID 9611
Full Name nuclear receptor corepressor 1
Alias N-CoR, N-CoR1, PPP1R109, TRAC1, hN-CoR
Introduction The most well characterized nuclear receptor corepressors are SMRT (silencing mediator for retinoic acid and thyroid hormone receptors) and its close paralog NCoR1 (nuclear receptor corepressor). NCoR1 functions to transcriptionally silence various unliganded, DNA bound non-steroidal nuclear receptors by serving as a large molecular scaffold that bridges the receptors with multiple chromatin remodeling factors that repress nuclear receptor-mediated gene transcription, in part, through deacetylation of core histones surrounding target promoters. Indeed, the N-terminal portion of NCoR1 possesses multiple distinct transcriptional repression domains (RDs) reponsible for the recruitment of additional components of the corepressor complex such as HDACs, mSin3, GPS2, and TBL1/TBLR1. In between the RDs lies a pair of potent repressor motifs known as SANT motifs (SWI3, ADA2, N-CoR, and TFIIIB), which recruit HDAC3 and histones to the repressor complex in order to enhance HDAC3 activity. The C-terminal portion of NCoR1 contains multiple nuclear receptor interaction domains (NDs), each of which contains a conserved CoRNR box (or L/I-X-X-I/V-I) motif that allow for binding to various unliganded nuclear hormone receptors such as thyroid hormone (THR) and retinoic acid (RAR) receptors.Recent genetic studies in mice have not only corroborated the wealth of biochemical studies involving NCoR1 but have also provided significant insight regarding the function of NCoR1 in mammalian development and physiology. Although it has been observed that loss of Ncor1 does not affect early embyonic development, likely due to compensation by Smrt, embryonic lethality ultimately results during mid-gestation, largely due to defects in erythropoesis and thymopoesis. Another study demonstrated that the NDs of NCoR1 are critical for its ability to function in a physiological setting as a transcriptional repressor of hepatic THR and Liver X Receptor (LXR).
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. E4S4N
Immunogen Recombinant protein specific to the carboxy terminus of human NCoR1 protein.
Source/Purification Antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human NCoR1 protein.
Usage
Application WB, IP, IF, IHC, ChIP
Dilutions Western Blot (1:1000); Immunoprecipitation (1:200)
MW(KDa) 270
Reactivity Human
Sensitivity Endogenous
Specificity It recognizes endogenous levels of total NCoR1 protein.
Storage & Handling
Storage Buffer Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide.
Preservative Less than 0.02% Sodium Azide
Storage Temp. Store at -20 °C.
Handling Do not aliquot the antibody.

For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.