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NCAM-1/CD56 Antibody
SPA-07849
| Size | Price |
| 20 µg | Online Inquiry |
| 0.1 mg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | NCAM-1 |
| Gene Abbr. | NCAM1 |
| Gene ID | 4684 |
| Full Name | neural cell adhesion molecule 1 |
| Alias | CD56, MSK39, NCAM |
| Introduction | CD56, a 175-220KDa glycoprotein, is a member of the Ig super family. It is expressed as three major isoforms and consists of five Ig-like domains and two Fibronectin-type III domains in the extracellular region. The 135kDa isoform is the basic molecule which is glycosylated or sialylated to produce the mature species. CD56 is widely expressed in nervous system, on NK cells and a specific set of Tcells. CD56+ NK cells and Tcells are unique in their ability to mediate cell-mediated cytotoxicity against certain tumor cell targets without MHC restriction. Other physiological functions of CD56 include mediating cell adhesion through homophilic and heterophilic interaction and activating intracellular signaling pathways resulting in neutrite extension and fasciculation, migration and synapses formation in brain. CD56 is also vital for neuronal development and plasticity in adult brain. It is used as a tumor marker in various cancers such as NK lymphomas and Merkel cell carcinoma. NCAM is expressed on most neuroectodermal derived lines, tissues, and neoplasms such as retinoblastoma, medulloblastoma, astrocytoma, and neuroblastoma. It is also expressed on some mesodermally derived tumors such as rhabdomyosarcoma and also on natural killer cells. |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | SPM489 |
| Isotype | IgG1 Kappa |
| Immunogen | Membrane preparation of a small cell lung carcinoma. |
| Usage | |
|---|---|
| Application | WB, FC, IF, IHC, IP |
| Dilutions | Western Blot (0.5-1 µg/mL); Flow Cytometry (0.5-1 µg/10^6 cells); Immunofluorescence (1-2 µg/mL); Immunoprecipitation (1-2 µg/500 µg) |
| Reactivity | Human |
| Specificity | This MAb reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas. |
| Storage & Handling | |
|---|---|
| Storage Buffer | 10 mM PBS with 0.05% BSA. |
| Preservative | 0.05% Sodium Azide |
| Storage Temp. | Store at 4 °C. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.