MyD88 Antibody - CD BioSciences

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MyD88 Antibody

MyD88 Antibody

SPA-07782

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Target Information
Target Name MyD88
Gene Abbr. MYD88
Gene ID 4615
Full Name MYD88 innate immune signal transduction adaptor
Alias IMD68, MYD88D
Introduction Members of the Toll-like receptor (TLR) family, named for the closely related Toll receptor in Drosophila, play a pivotal role in innate immune responses. TLRs recognize conserved motifs found in various pathogens and mediate defense responses. Triggering of the TLR pathway leads to the activation of NF-κB and subsequent regulation of immune and inflammatory genes. The TLRs and members of the IL-1 receptor family share a conserved stretch of approximately 200 amino acids known as the Toll/Interleukin-1 receptor (TIR) domain. Upon activation, TLRs associate with a number of cytoplasmic adaptor proteins containing TIR domains, including myeloid differentiation factor 88 (MyD88), MyD88-adaptor-like/TIR-associated protein (MAL/TIRAP), Toll-receptor-associated activator of interferon (TRIF), and Toll-receptor-associated molecule (TRAM). This association leads to the recruitment and activation of IRAK1 and IRAK4, which form a complex with TRAF6 to activate TAK1 and IKK.Activation of IKK leads to the degradation of IκB, which normally maintains NF-κB in an inactive state by sequestering it in the cytoplasm.MyD88 was originally isolated as a myeloid differentiation primary response gene that is rapidly induced upon IL-6 stimulated differentiation of M1 myeloleukemic cells into macrophages. It contains an amino-terminal death domain separated from a carboxyl-terminal TIR domain and functions as an adaptor in TLR/IL-1 receptor signaling. The death domain of MyD88 mediates interactions with the IRAK complex triggering a signaling cascade that includes the activation of NF-κB.
Product Details
Host Goat
Clonality Polyclonal
Clone No. N/A
Isotype IgG
Immunogen E. coli-derived recombinant human MyD88. Met1-Pro296. Q99836.
Usage
Application WB, FC, IF
Dilutions Western Blot (0.5 µg/mL); Immunofluorescence (5-15 µg/mL); Flow Cytometry (2.5 µg/10^6 cells)
MW(KDa) 33
Reactivity Human
Specificity Detects human MyD88 in direct ELISAs and Western Blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant mouse MyD88 is observed.
Storage & Handling
Storage Buffer Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
Preservative No Preservative
Storage Temp. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Handling Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

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