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MKK3/MEK3 Antibody
SPA-07615
| Size | Price |
| 25 µg | Online Inquiry |
| 100 µg | Online Inquiry |
| More Options | Online Inquiry |
| Target Information | |
|---|---|
| Target Name | MKK3 |
| Gene Abbr. | MAP2K3 |
| Gene ID | 5606 |
| Full Name | mitogen-activated protein kinase kinase 3 |
| Alias | MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2 |
| Introduction | MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase. MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines. Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr222 on MKK3 and Ser207 and Thr211 on MKK6.Three alternatively spliced transcript variants of MKK3 encoding distinct isoforms have been reported. Isoform B utilizes a different start codon compared to isoform C resulting in the production of a N-terminal segment of isoform B which is shorter and distinct from isoform C. MKK3b is the predominant form of MKK3 and strongly activates p38 MAP kinase (6) |
| Product Details | |
|---|---|
| Host | Mouse |
| Clonality | Monoclonal |
| Clone No. | 275909 |
| Isotype | IgG2B |
| Immunogen | E. coli-derived recombinant human MKK3. Met1-Ser347. P46734. |
| Usage | |
|---|---|
| Application | WB, IF |
| Dilutions | Western Blot (1 µg/mL); Immunofluorescence (15-30 µg/mL) |
| MW(KDa) | 40 |
| Reactivity | Human, Mouse, Rat |
| Specificity | Detects human MKK3 in direct ELISAs and Western Blots. In Western Blots, no cross-reactivity with recombinant human MKK6 is observed. |
| Storage & Handling | |
|---|---|
| Storage Buffer | Supplied as a 0.2 μm filtered solution in PBS. |
| Preservative | No Preservative |
| Storage Temp. | 12 months from date of receipt, -20 to -70 °C, as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. 6 months, -20 to -70 °C under sterile conditions after opening. |
| Handling | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
For research use only. Not intended for any clinical use. No products from CD BioSciences may be resold, modified for resale or used to manufacture commercial products without prior written approval from CD BioSciences.