MKK3/MEK3 Antibody - CD BioSciences

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MKK3/MEK3 Antibody

MKK3/MEK3 Antibody

SPA-07608

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Target Information
Target Name MKK3
Gene Abbr. MAP2K3
Gene ID 5606
Full Name mitogen-activated protein kinase kinase 3
Alias MAPKK3, MEK3, MKK3, PRKMK3, SAPKK-2
Introduction MKK3 and MKK6 are two closely related dual-specificity protein kinases that activate p38 MAP kinase. MKK3 and MKK6 both phosphorylate and activate p38 MAP kinase at its activation site, Thr-Gly-Tyr, but do not phosphorylate or activate Erk1/2 or SAPK/JNK. Phosphorylation of p38 MAP kinase dramatically stimulates its ability to phosphorylate protein substrates such as ATF-2 and Elk-1. MKK3 and MKK6 are both activated by different forms of cellular stress and inflammatory cytokines. Activation of MKK3 and MKK6 occurs through phosphorylation at Ser189 and Thr222 on MKK3 and Ser207 and Thr211 on MKK6.Three alternatively spliced transcript variants of MKK3 encoding distinct isoforms have been reported. Isoform B utilizes a different start codon compared to isoform C resulting in the production of a N-terminal segment of isoform B which is shorter and distinct from isoform C. MKK3b is the predominant form of MKK3 and strongly activates p38 MAP kinase (6)
Product Details
Host Rabbit
Clonality Monoclonal
Clone No. SD20-93
Isotype IgG
Immunogen Recombinant Protein.
Usage
Application WB, FC, IF, IHC, IP
Dilutions Western Blot (1:100-1:2000); Flow Cytometry (1:10-1:1000); Immunofluorescence (1:10-1:500); Immunohistochemistry (1:10-1:500)
MW(KDa) 40
Reactivity Human, Mouse, Rat
Storage & Handling
Storage Buffer TBS (pH 7.4), 1% BSA, 40% Glycerol.
Preservative 0.05% Sodium Azide
Storage Temp. Store at 4 °C for a short term. Aliquot and store at -20 °C for long-term storage.
Handling Avoid freeze-thaw cycles.

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